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重要文件

G4509

Sigma-Aldrich

甘油激酶 来源于大肠杆菌

lyophilized powder, 40-100 units/mg protein

同義詞:

ATP:甘油3-磷酸转移酶, 甘油激酶

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.54

生物源

Escherichia coli

品質等級

形狀

lyophilized powder

比活性

40-100 units/mg protein

成份

Protein, ≥50% biuret

異物活動

Triose-phosphate isomerase, hexokinase, myokinase, glycerol dehydrogenase and NADH oxidase ≤0.05%

儲存溫度

−20°C

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應用

利用甘油激酶(glpK)研究疼痛调控神经肽对人脂肪细胞脂解的影响。

生化/生理作用

甘油激酶催化甘油的tge MgATP依赖性磷酸化以产生sn-甘油-3-磷酸,并且是甘油利用中的限速酶。 它也受到果糖-1,6-二磷酸的反馈调节。
甘油激酶催化甘油的tge MgATP依赖性磷酸化以产生sn-甘油-3-磷酸,并且是甘油利用中的限速酶。 <<<20,21>>> 它也受到果糖-1,6-二磷酸的反馈调节。<<<22>>>

單位定義

一个单位为在pH 9.8和25℃下,在PK/LDH的偶联体系内每分钟将1.0 μmole甘油和ATP转化为L-α甘油磷酸盐和ADP。

外觀

部分纯化的冻干粉末,平衡主要是盐和EDTA。

象形圖

Health hazard

訊號詞

Danger

危險聲明

防範說明

危險分類

Resp. Sens. 1

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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V Van Harmelen et al.
The Journal of biological chemistry, 274(26), 18243-18251 (1999-06-22)
The effects of acylation-stimulating protein (ASP) and insulin on free fatty acid (FFA) release from isolated human fat cells and the signal transduction pathways to induce these effects were studied. ASP and insulin inhibited basal and norepinephrine-induced FFA release by
A Girard et al.
The Biochemical journal, 274 ( Pt 3), 819-824 (1991-03-15)
An artificial-membrane-bound glycerokinase chosen as a membrane-bound two-substrate-enzyme model has been used to separate two unequal compartments of a specially designed diffusion cell. An interesting feature is the asymmetry of compartments and the existence of a diffusion layer adjacent to
V van Harmelen et al.
International journal of obesity (2005), 34(8), 1333-1340 (2010-03-17)
Neuropeptides NPFF and NPSF are involved in pain control, acting through the G-protein coupled receptors (GPR)74 (high affinity for NPFF) and GPR147 (equal affinity for NPFF and NPSF). GPR74 also inhibits catecholamine-induced adipocyte lipolysis and regulates fat mass in humans.
Ioanna Gouni-Berthold et al.
The British journal of nutrition, 107(11), 1694-1706 (2011-10-15)
Animal and human studies suggest that a malleable protein matrix (MPM) from whey decreases plasma lipid concentrations and may positively influence other components of the metabolic syndrome such as glucose metabolism and blood pressure (BP). The primary objective of this
Daria V Cherkashina et al.
Rejuvenation research, 14(6), 661-667 (2011-10-20)
Abstract Effects of stem and progenitor cells or their compounds on recipient cells are investigated intensively today. In spite of this, their ability to interact with native cells and the final targets affected by them, particularly biochemical parameters that characterize

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Enzymatic assay of lipase type XIII from Pseudomonas sp. using a coupled enzyme system of glycerol kinase and glycerophosphate oxidase (EC 3.1.1.3)

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