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Key Documents

FG1215

Sigma-Aldrich

RPMI-1640 Medium

With stable glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

同義詞:

Roswell Park Memorial Institute 1640 medium

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About This Item

分類程式碼代碼:
12352207
NACRES:
NA.71

product name

RPMI-1640 Medium, With L-alanyl-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered
sterile

形狀

liquid

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

sodium pyruvate: no
NaHCO3: yes
HEPES: no
phenol red: yes
stable glutamine: yes

運輸包裝

ambient

儲存溫度

2-8°C

一般說明

RPMI 1640 Medium was developed at Roswell Park Memorial Institute in 1966 by Moore and his co-workers. A modification of McCoy′s 5A Medium, it was formulated to support lymphoblastoid cells in suspension culture, but it has since been shown to support a wide variety of cells that are anchorage dependent. Originally intended to be used with a serum supplement, RPMI 1640 has been shown to support several cell lines in the absence of serum. It has also been widely used in fusion protocols and in the growth of hybrid cells.

應用

RPMI-1640 Medium has been used to culture murine fibroblast cells. It has also been used to culture human ovarian and rat substantia nigra cells.

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 1

閃點(°F)

Not applicable

閃點(°C)

Not applicable


分析證明 (COA)

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M Granados et al.
Journal of cardiovascular translational research, 10(4), 374-390 (2017-05-04)
Decellularized scaffolds represent a promising alternative for mitral valve (MV) replacement. This work developed and characterized a protocol for the decellularization of whole MVs. Porcine MVs were decellularized with 0.5% (w/v) SDS and 0.5% (w/v) SD and sterilized with 0.1%

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