FG0435

DMEM - high glucose

With stable glutamine, and sodium bicarbonate, without sodium pyruvate, liquid, sterile-filtered, suitable for cell culture

• 同義詞: DME, Dulbecco′s Modified Eagle′s Medium - high glucose
• 分類程式碼代碼: 12352207
• NACRES: NA.71

屬性

• 產品名稱: Dulbecco′s Modified Eagle′s Medium - high glucose, With 4500 mg/L glucose, L-alanyl-glutamine, and sodium bicarbonate, without sodium pyruvate., liquid, sterile-filtered, suitable for cell culture
• 品質等級: 400
• 無菌: sterile-filtered; sterile
• 形狀: liquid
• 技術: cell culture | mammalian: suitable
• 成分: phenol red: yes; glucose: high; stable glutamine: yes; sodium pyruvate: no; HEPES: no; NaHCO₃: yes
• 運輸包裝: ambient
• 儲存溫度: 2-8°C

描述

一般說明

This DMEM-Hi glucose medium differs from the original formula, because it is supplemented with L-alanyl-L-glutamine dipeptide. Glutamine is notoriously unstable in cell culture. This dipeptide provides a more stable form of glutamine for use in cell cultures. It also differs from the original DMEM-Hi formulation wherein pyridoxine is substituted for pyridoxal. Pyridoxal is an unstable component of media.

應用

Dulbecco′s Modified Eagle′s Medium (DMEM) is a modification of Basal Medium Eagle (BME) that contains four-fold concentrations of the amino acids and vitamins. The original formulation contained 1000 mg/L of glucose and was used to culture embryonic mouse cells. Since then, it has been modified in several ways to support primary cultures of mouse and chicken cells, as well as a variety of normal and transformed cells. Each of these media offers a different combination of L-glutamine and sodium pyruvate. Additionally, the glucose levels have been raised to 4500 mg/L, contributing to the name "DMEM/High".

安全資訊

• 儲存類別代碼: 12 - Non Combustible Liquids
• 水污染物質分類（WGK）: WGK 1
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable