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E7885

Sigma-Aldrich

弹性蛋白酶 来源于猪胰腺

lyophilized powder, suitable for cell culture

同義詞:

弹性蛋白酶 来源于猪胰腺, 胰酞酶 E

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About This Item

CAS號碼:
酶委員會編號:
EC號碼:
MDL號碼:
分類程式碼代碼:
12352204
NACRES:
NA.75

生物源

Porcine

形狀

lyophilized powder

比活性

≥4 units/mg protein

分子量

25.9 kDa

包裝

pkg of 1 μmol

技術

cell culture | mammalian: suitable

pH值

8

適合性

suitable for

異物活動

Trypsin ≤50 BAEE units/mg protein

運輸包裝

dry ice

儲存溫度

−20°C

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相關類別

一般說明

弹性蛋白酶是 240 个氨基酸残基的单一多肽链,含有四个二硫键。分子量约为 25.9kDa。该酶被合成为无活性的酶原,原弹性蛋白酶,其通过胰蛋白酶在 N-末端的有限蛋白水解而转化为活性形式。

應用

来自猪胰腺的弹性蛋白酶用于以下应用:
  • 细胞培养技术
  • ELISA 技术
  • 用于测定弹性蛋白酶抑制活性

生化/生理作用

弹性蛋白酶水解弹性蛋白(弹性纤维的特定蛋白质)并消化血红蛋白、酪蛋白和纤维蛋白。
降低弹性蛋白的酶,弹性蛋白是参与拉伸过程的组织的基质成分。它是一种具有广泛特异性的丝氨酸蛋白酶。它切割小疏水性氨基酸(如 Ile、Gly、Ala、Ser、Val 和 Leu)的羧基侧的蛋白质。该酶还水解酰胺和酯,例如 N-苯甲酰基-L-丙氨酸甲酯。

包裝

包装尺寸基于蛋白质含量

單位定義

一个单元将在 25℃ 下,每分钟,pH8.0,水解 1.0 μmole 的 N-琥珀酰-L-Ala-Ala-Ala-对硝基苯胺。

象形圖

Health hazardExclamation mark

訊號詞

Danger

危險分類

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

標靶器官

Respiratory system

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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P Hamet et al.
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The amino acid sequence of elastase inhibitor, AFLEI, isolated from Aspergillus flavus was determined by the Edman sequencing procedure of peptides derived from digests utilizing clostripain. A molecular weight of 7,525.8 was observed by TOF-MS. AFLEI contained 68 amino acid
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條款

Enzymatic Assay of Elastase (EC 3.4.21.36), a continuous spectrophotometric rate determination.

Chromatograms

application for HPLC

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