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E7408

Sigma-Aldrich

p3XFLAG-CMV™-7 Expression Vector

shuttle vector for transient expression of N-terminal Met-3xFLAG

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About This Item

分類程式碼代碼:
12352200

籤條

Met-3X FLAG tagged

等級

for molecular biology

形狀

buffered aqueous solution

肽標籤位置

N-terminal

運輸包裝

dry ice

儲存溫度

−20°C

一般說明

p3XFLAG-CMV™-7 Expression Vector is a 4.7 kb derivative of pCMV5 used to establish expression of N-terminal Met-FLAG® fusion proteins in mammalian cells. The vector encodes the ANTI-FLAG M2 antibody epitope (Asp-Tyr-Lys-Xaa-Xaa-Asp) three times, upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2. The third epitope includes the enterokinase recognition sequence allowing cleavage of the 3XFLAG peptide from the purified fusion protein. Efficiency of replication is optimal when using an SV40 T antigen-expressing host.

p3XFLAG-CMV-7-BAP Control Plasmid is a 6.2 kb derivative of pCMV5 used for transient intracellular expression of N-terminal 3XFLAG bacterial alkaline phosphatase fusion protein in mammalian cells. The vector encodes three adjacent FLAG epitopes (Asp- Tyr-Lys-Xaa-Xaa-Asp) upstream of the multiple cloning region. This results in increased detection sensitivity using ANTI-FLAG M2 antibody.3 The third FLAG epitope includes the enterokinase recognition sequence, allowing cleavage of the 3XFLAG peptide from the purified fusion protein.

Vector Maps and Sequences

成分

  • p3XFLAG-CMV™-7 Expression Vector 20 μg (E2400) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
  • p3XFLAG-CMV™-7-BAP Control Plasmid 20 μg (C7472) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

原則

The promoter-regulatory region of the human cytomegalovirus drives transcription of FLAG®-fusion constructs. p3XFLAG-CMV™-7 Expression Vector is a shuttle vector for E. coli and mammalian cells.

法律資訊

This product is covered by the following patents owned by Sigma-Aldrich Co. LLC: US6,379,903, US7,094,548, JP4405125,EP1220933, CA2386471 and AU774216.
3xFLAG is a trademark of Sigma-Aldrich Co. LLC
FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany
p3xFLAG-CMV is a trademark of Sigma-Aldrich Co. LLC

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儲存類別代碼

10 - Combustible liquids

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Journal of virology, 79(6), 3525-3535 (2005-02-26)
The murine cytomegalovirus (MCMV) proteins encoded by US22 genes M139, M140, and M141 function, at least in part, to regulate replication of this virus in macrophages. Mutant MCMV having one or more of these genes deleted replicates poorly in macrophages
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