E4781

Anti-eRF3a/GSPT1 兔抗

~1 mg/mL, affinity isolated antibody, buffered aqueous solution

• 同義詞: Anti-Eukaryotic Release Factor 3a, Anti-G1 to S phase transition 1, Anti-GST1, homolog of yeast
• MDL號碼: MFCD09265357
• 分類程式碼代碼: 12352203
• NACRES: NA.41

屬性

• 生物源: rabbit
• 共軛: unconjugated
• 抗體表格: affinity isolated antibody
• 抗體產品種類: primary antibodies
• 無性繁殖: polyclonal
• 形狀: buffered aqueous solution
• 分子量: antigen 76.5 kDa
• 物種活性: human
• 包裝: antibody small pack of 25 μL
• 濃度: ~1 mg/mL
• 技術: immunoprecipitation (IP): 1-2 μg using HeLa cell lysates; indirect immunofluorescence: 5-10 μg/mL using paraformadehyde-fixed HeLa cells; western blot: 0.5-1 μg/mL using HeLa cell lysates
• UniProt登錄號: P15170
• 運輸包裝: dry ice
• 儲存溫度: −20°C
• 目標翻譯後修改: unmodified
• 基因資訊: human ... GSPT1(2935) ; mouse ... Gspt1(14852) ; rat ... Gspt1(24420)

描述

一般說明

Eukaryotic release factor 3 a (eRF3a) is encoded by the gene G1 to S phase transition 1 (GSPT1) in humans. The eRF3a/GSPT1 exon 1 is a translation termination protein characterized with a tri-nucleotide GGC repeat coding for a polyglycine expansion in the amino-terminal end.

免疫原

synthetic peptide corresponding to amino acids 190-204 of human eRF3a/GSPT1, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse.

應用

Anti-eRF3a/GSPT1 antibody produced in rabbit has been used in immunoprecipitation, indirect immunofluorescence and western blotting.

生化／生理作用

Eukaryotic release factor 3 a (eRF3a) is proteolytically processed into an IAP (Inhibitors of Apoptosis Proteins)-binding protein. Processed eRF3a interacts with IAPs and can promote caspase activation, IAP ubiquitination and apoptosis. The IAP binding motif is exposed only after proteolytic cleavage of a 69-residue leader sequence. eRF3a also affects mRNA stability and translation initiation by interacting with Poly(A)-binding protein (PABP) and the surveillance factor Upf1.

Eukaryotic release factor 3a (eRF3a) is a translation termination protein that is encoded by G1 to S phase transition 1 gene (GSPT1) in humans. The eRF3a/GSPT1 exon 1 contains a tri-nucleotide GGC repeat coding for a polyglycine expansion in the N-terminal of the protein. It is associated with protein translation as a regulator of apoptosis signal-regulating kinase 1 (ASK1). GSPT1 interacts with ASK1 and enhances ASK1-induced apoptotic activity by activating caspase-3. It plays a novel role in the regulation of ASK1-mediated apoptosis. GSPT1 is found to be up-regulated in various human cancers and is considered as a potential proto-oncogene.

外觀

0.01M 磷酸缓冲盐溶液，pH 7.4，含 15mM 叠氮化钠。

免責聲明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids
• 水污染物質分類（WGK）: WGK 3
• 閃點（°F）: Not applicable
• 閃點（°C）: Not applicable
• 個人防護裝備: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)