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應用
来自Sigma的酶被用来激活酶原，以检测胰蛋白酶的活性。研究胰蛋白酶原中未配对半胱氨酸的结构和进化结果。该产品已用于测量胰蛋白酶，同时研究农药诱导的基因表达改变在美洲螯龙虾中的影响。Sigma的酶已用于开发一种新的测定方法，在脂结合apoA-I存在的情况下，测量无脂apoA-I的水平。肠肽酶可以特异性切割人的无脂apoA-I，但不能切割apoA-I的脂质结合形式，从而产生22 kDa的N端片段。还用它研究了钙和植酸对胰蛋白酶原激活和胰蛋白酶稳定性的影响。
来自猪肠的肠激酶已用于研究大鼠肠肽酶的互补DNA克隆和测序。来自猪肠的肠激酶也用于了解胃抑制多肽的胰岛素性区域。
生化／生理作用
肠激酶是一种膜结合的丝氨酸蛋白酶，可特异性和快速地将胰蛋白酶原转化为胰蛋白酶，从而触发其他酶原向活性酶的转化。它的分子量约为150 kDa。该酶是由35-47 kDa亚基组成的异二聚体。轻链和重链通过两个二硫键连接。它是一种含有35%碳水化合物的糖蛋白。胰蛋白酶原的多肽链仅在-（Asp）4-Lys-序列后水解。该酶被大豆胰蛋白酶抑制剂抑制。肠激酶通常用于蛋白修饰和氨基酸序列测定。
單位定義
在pH 5.6，25℃条件下，一个单位每分钟将从胰蛋白酶原产生1.0纳摩尔的胰蛋白酶。
外觀
含有磷酸钠缓冲盐的冻干粉
儲存類別代碼
11 - Combustible Solids
水污染物質分類（WGK）
WGK 3
閃點（°F）
Not applicable
閃點（°C）
Not applicable
個人防護裝備
Eyeshields, Gloves, type N95 (US)
分析證明 (COA)
輸入產品批次/批號來搜索 分析證明 (COA)。在產品’s標籤上找到批次和批號，寫有 ‘Lot’或‘Batch’.。
W Safi et al.
Journal of lipid research, 42(5), 864-872 (2001-05-16)
Recent studies indicate that certain lipid-poor forms of apolipoprotein (apo)A-I may be particularly important in promoting cholesterol release from overburdened cells in the periphery. However, a detailed understanding of the physiological relevance of these species has been hampered by the
Erzsébet Kénesi et al.
Biochemical and biophysical research communications, 309(4), 749-754 (2003-09-19)
Vertebrate trypsins usually contain six disulfide bonds but human trypsin 1 (PRSS1) contains only five and human trypsin 2 (PRSS2) contains only four. To elucidate possible evolutionary pathways leading to the loss of disulfide bonds, we have constructed mutants lacking
Michael N Horst et al.
Comparative biochemistry and physiology. Part D, Genomics & proteomics, 2(1), 44-52 (2007-03-01)
Using subtractive hybridization, we have identified 17 genes that are either up- or down-regulated in the hepatopancreas (Hp) of the lobster, Homarus americanus, by acute exposure to the juvenile hormone analog methoprene. The expression of some of the genes obtained
Effect of calcium and phytic acid on the activation of trypsinogen and the stability of trypsin.
Caldwell RA
Journal of Agricultural and Food Chemistry, 40 (1), 43-46 (1992)
G W Morrow et al.
Canadian journal of physiology and pharmacology, 74(1), 65-72 (1996-01-01)
Glucose-dependent insulinotropic polypeptide or gastric inhibitory polypeptide (GIP) is a 42 amino acid intestinal hormone, which exhibits several direct and indirect effects on fat and glucose metabolism. To determine the bioactive region(s) of the molecule, synthetic and proteolytic fragments of
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