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D8273

Sigma-Aldrich

脱氧核糖核酸−纤维素单链 来源于小牛胸腺 DNA

lyophilized powder

同義詞:

单链DNA纤维素, DNA-纤维素

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About This Item

MDL號碼:
分類程式碼代碼:
41106305
eCl@ss:
32160414
NACRES:
NA.51

一般說明

小牛胸腺 DNA 单链脱氧核糖核酸−纤维素又称ctDNA,是一种用于 DNA 结合剂研究的天然 DNA 来源。 ctDNA 的检测限约为 2.0ng,光散射强度(I(LS))的线性范围为 0.05-1.5mµg。

應用

小牛胸腺 DNA 脱氧核糖核酸−纤维素单链用于研究多种蛋白质、核苷酸和化学试剂(包括抗癌药物)的结合参数和结合特异性。 它常用于pull-down分析和色谱程序。

品質

可能含有微量的 Tris 和 EDTA。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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S S Zhang et al.
The Journal of biological chemistry, 266(30), 20483-20490 (1991-10-25)
Two DNA helicases from calf thymus nuclei have been purified and characterized. The two proteins, designated as nuclear DNA helicase I and II, were copurified on Bio-Rex 70, DEAE-Sepharose, phosphocellulose and subsequently separated from each other on a heparin-Sepharose column.
A Georgaki et al.
Nucleic acids research, 22(7), 1128-1134 (1994-04-11)
A DNA helicase from calf thymus, called DNA helicase F, copurified with replication protein A through several steps of purification including DEAE-Sephacel, hydroxyapatite and single stranded DNA cellulose. It is finally separated from replication protein A on FPLC Mono Q
T Kuzuhara et al.
Biochemical and biophysical research communications, 362(4), 805-810 (2007-09-04)
Tumor necrosis factor-alpha (TNF-alpha) inducing protein (Tipalpha) is a carcinogenic factor secreted from Helicobacter pylori (H. pylori), mediated through both enhanced expression of TNF-alpha and chemokine genes and activation of nuclear factor-kappaB. Since Tipalpha enters gastric cancer cells, the Tipalpha
D Pisetsky et al.
The Journal of rheumatology, 26(9), 1934-1938 (1999-09-24)
To elucidate the epitope structure to DNA by identifying antigenic determinants on bacterial DNA bound by anti-DNA antibodies from normal human subjects (NHS) and patients with systemic lupus erythematosus (SLE). Sera from NHS and patients with SLE were tested by
Q Hua et al.
Cellular and molecular life sciences : CMLS, 60(2), 413-421 (2003-04-08)
Tau, a major microtubule-associated protein of the neuron, which is known to promote the assembly of and to stabilize microtubules, has also been seen associated with chromatin in neuronal cell lines, but its role in this subcellular compartment is still

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