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重要文件

D2629

Sigma-Aldrich

6-(二甲氨基)嘌呤

≥98%

同義詞:

6-DMAP, N6,N6-二甲腺嘌呤

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About This Item

經驗公式(希爾表示法):
C7H9N5
CAS號碼:
分子量::
163.18
Beilstein:
7634
EC號碼:
MDL號碼:
分類程式碼代碼:
41106305
PubChem物質ID:
NACRES:
NA.51

生物源

synthetic (organic)

化驗

≥98%

形狀

powder

mp

259-262 °C (lit.)

溶解度

water: 50 mg/mL, clear to hazy, colorless to light yellow

儲存溫度

−20°C

SMILES 字串

CN(C)c1ncnc2[nH]cnc12

InChI

1S/C7H9N5/c1-12(2)7-5-6(9-3-8-5)10-4-11-7/h3-4H,1-2H3,(H,8,9,10,11)

InChI 密鑰

BVIAOQMSVZHOJM-UHFFFAOYSA-N

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一般說明

6-二甲氨基嘌呤(6-DMAP)是一种嘌呤类代谢物,含有2个缩合杂环及与腺嘌呤的嘌呤氨基(环外氨基)连接的2个甲基。

應用

6-二甲氨基嘌呤已用于:
  • 作为GR-1 aa培养基(牛培养基)补充剂,用于牛卵母细胞的孤雌激活,以研究其对胚胎发育的影响
  • 核移植胚胎生产中的激活步骤
  • 作为HCR2aa培养基补充剂,用于激活通过异种体细胞核移植(iSCNT)技术获得的异种胚胎

生化/生理作用

6-二甲氨基嘌呤(6-DMAP)是一种蛋白激酶和细胞周期蛋白依赖性激酶抑制剂。它是一种次生代谢产物,介导RNA修饰。6-DMAP是一种强效的细胞质分裂抑制剂,用于孤雌生殖和减数分裂研究。它也可用于促进哺乳动物卵母细胞的前核形成。根据飞秒荧光上转换光谱技术,6-DMAP是一种双荧光(dual fluorescence)分子。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


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Gaid Le Beux et al.
Theriogenology, 60(6), 1049-1058 (2003-08-26)
Improvement of the ability to maintain germinal vesicle stage oocytes in vitro is important for the acquisition of developmental competence. Maintaining oocytes at this stage without damaging their quality would allow synchronization of maturation and homogenization of the oocytes population.
Karen Versieren et al.
Fertility and sterility, 96(3), 624-628 (2011-07-22)
To create a pool of frozen donated human oocytes and find the optimal stage for slow controlled-rate freezing of human in vitro matured oocytes. Oocytes at different developmental stages of maturation (germinal vesicle, metaphase I, or metaphase II) and oocytes that
De Cheng et al.
PloS one, 7(12), e51778-e51778 (2012-12-20)
Porcine induced pluripotent stem (piPS) cell lines have been generated recently by using a cocktail of defined transcription factors, however, the features of authentic piPS cells have not been agreed upon and most of published iPS clones did not meet
Marcin Szpila et al.
Scientific reports, 9(1), 11859-11859 (2019-08-16)
Postovulatory ageing of mammalian oocytes occurs between their ovulation and fertilization and has been shown to decrease their developmental capabilities. Aged oocytes display numerous abnormalities, including altered Ca2+ signalling. Fertilization-induced Ca2+ oscillations are essential for activation of the embryonic development
Justine Collier
Current opinion in microbiology, 12(6), 722-729 (2009-09-29)
N(6)-methyl-adenines can serve as epigenetic signals for interactions between regulatory DNA sequences and regulatory proteins that control cellular functions, such as the initiation of chromosome replication or the expression of specific genes. Several of these genes encode master regulators of

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