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重要文件

D2429

Sigma-Aldrich

DMEM - low glucose

With sodium pyruvate, without ʟ-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable fore cell culture

同義詞:

DME, DMEM

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About This Item

MDL號碼:
分類程式碼代碼:
12352207
NACRES:
NA.75

產品名稱

杜氏改良 Eagle 培养基-低葡萄糖, 10 ×, With 1000 mg/L glucose (1x), without L-glutamine, sodium bicarbonate, and folic acid, liquid, sterile-filtered, suitable for cell culture

品質等級

無菌

sterile-filtered

形狀

liquid

濃度

10 ×

技術

cell culture | mammalian: suitable

雜質

endotoxin, tested

成分

NaHCO3: no
sodium pyruvate: yes
phenol red: yes
glucose: low
L-glutamine: no
HEPES: no

運輸包裝

ambient

儲存溫度

2-8°C

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一般說明

杜氏改良Eagle培养基(DME)是对Eagle基础培养基(BME)的改良版,含有高四倍浓度的氨基酸和维生素及其他添加成分。据报道,原本的DME配方适合用于小鼠胚胎细胞培养,含有1,000 mg/L葡萄糖。

應用

杜氏改良Eagle培养基(低糖)已用于:
  • 在类器官包埋时,中和胶原蛋白I溶液
  • 黑色素瘤细胞系培养
  • 癌组织样本保存
  • C2C12细胞分化培养基

重構

补充 3.7 g/L 碳酸氢钠,0.584 g/L L -谷氨酰胺,0.004 g/L 叶酸,以 1×。

象形圖

Exclamation mark

訊號詞

Warning

危險聲明

危險分類

Skin Sens. 1

儲存類別代碼

12 - Non Combustible Liquids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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分析證明 (COA)

Lot/Batch Number

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Folic acid supplements taken during pregnancy can prevent neural tube defects and other developmental abnormalities. Here, we explored the effects of folate supplementation on gene expression and DNA methylation during C2C12 differentiation. Based on the folic acid concentration, this study
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