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Key Documents

D2001

Sigma-Aldrich

脱氧核糖核酸 钠盐 来源于大肠杆菌 菌株 B

Type VIII

同義詞:

DNA

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About This Item

CAS號碼:
EC號碼:
MDL號碼:
分類程式碼代碼:
41106305
eCl@ss:
32160414
NACRES:
NA.51

種類

Type VIII

品質等級

形狀

fibers

雜質

<2% protein

儲存溫度

2-8°C

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應用

大肠杆菌DNA在研究中具有多种用途。 大肠杆菌DNA(ecDNA)可用于研究能够调节DNA结构和功能的DNA结合剂。 大肠杆菌DNA可用于溶液中DNA行为的物理化学研究,并评估物种特异性DNA的结构和结合结构域。该DNA对于基因组分析,包括PCR、噬菌体λ中的文库构建以及异源预杂交和杂交实验方案特别有用。
来自大肠杆菌B菌株的脱氧核糖核酸钠盐已被用于测量解链温度(Tm)和差异扫描量热法(DSC)研究中。它也已被用作聚合酶链反应(PCR)中的模板序列。

準備報告

按照Marmur, J., J. Mol. Biol., 3, 208 (1961)中的过程制备而得。

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, type N95 (US)


分析證明 (COA)

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R Richins et al.
Biotechnology progress, 17(2), 252-257 (2001-04-21)
The Escherichia coli DNA binding protein FIS is a transcriptional modulator involved in the regulation of many cellular processes, including the activation of rRNA synthesis. High-level overproduction of FIS in early, mid, or late log cultures resulted in growth-phase- and
William G Dundon et al.
International journal of medical microbiology : IJMM, 291(6-7), 545-550 (2002-03-14)
Infection of the stomach mucosa by the gastric pathogen Helicobacter pylori is accompanied by a large infiltration of neutrophils and monocytes which are believed to contribute substantially to H. pylori-induced gastritis. A protein was identified (HP-NAP for neutrophil-activating protein from
Geoffrey S Briggs et al.
The Journal of biological chemistry, 282(17), 12353-12357 (2007-02-20)
The DNA-binding protein, RdgC, is associated with recombination and replication fork repair in Escherichia coli and with the virulence-associated, pilin antigenic variation mediated by RecA and other recombination proteins in Neisseria species. We solved the structure of the E. coli
Mitsumasa Hashimoto et al.
The Journal of biological chemistry, 278(31), 28501-28507 (2003-05-16)
Closely opposed lesions form a unique class of DNA damage that is generated by ionizing radiation. Improper repair of closely opposed lesions could lead to the formation of double strand breaks that can result in increased lethality and mutagenesis. In
Differential scanning calorimetry of bacteria
Miles CA, et al.
Microbiology, 132(4), 939-952 (1986)

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