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Key Documents

D0942

Sigma-Aldrich

Anti-DHFR, C-terminal antibody produced in rabbit

1.0-1.5 mg/mL, affinity isolated antibody, buffered aqueous solution

同義詞:

Anti-Dihydrofolate reductase

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About This Item

MDL號碼:
分類程式碼代碼:
12352203
NACRES:
NA.56

生物源

rabbit

共軛

unconjugated

抗體表格

affinity isolated antibody

抗體產品種類

primary antibodies

無性繁殖

polyclonal

形狀

buffered aqueous solution

物種活性

mouse, human, rat

濃度

1.0-1.5 mg/mL

技術

immunoprecipitation (IP): 0.5-1.0 μg using 100-200 ng of purified DHFR
western blot (chemiluminescent): 0.5-1.0 μg/mL using 50-100 ng of purified recombinant DHFR, or 293T human embryonic kidney cell extract, or Jurkat human acute T Cell leukemia cell extract

UniProt登錄號

運輸包裝

dry ice

儲存溫度

−20°C

目標翻譯後修改

unmodified

基因資訊

human ... DHFR(1719)
mouse ... Dhfr(13361)
rat ... Dhfr(24312)

特異性

DHFR and DHFR fusion proteins. The epitope resides within amino acids 171-185 of mouse DHFR.

免疫原

amino acids 171-185 of mouse DHFR, conjugated to KLH via an N-terminal added cysteine residue.

外觀

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

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儲存類別代碼

10 - Combustible liquids

水污染物質分類(WGK)

WGK 2

閃點(°F)

Not applicable

閃點(°C)

Not applicable

個人防護裝備

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


分析證明 (COA)

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Soledad Chazarra et al.
Applied biochemistry and biotechnology, 162(7), 1834-1846 (2010-04-16)
Recent reports describe the inhibition of human dihydrofolate reductase (hDHFR) by natural tea polyphenols. This finding could explain the epidemiologic data on their prophylactic effects for certain forms of cancer, and it raises the possibility that natural and synthetic polyphenols
Gilad Barshad et al.
Scientific reports, 9(1), 9987-9987 (2019-07-12)
Mitochondrial complex I (CI) is the largest multi-subunit oxidative phosphorylation (OXPHOS) protein complex. Recent availability of a high-resolution human CI structure, and from two non-human mammals, enabled predicting the impact of mutations on interactions involving each of the 44 CI
Stephen W Michnick et al.
Methods in molecular biology (Clifton, N.J.), 756, 395-425 (2011-08-27)
Protein-fragment Complementation Assays (PCAs) are a family of assays for detecting protein-protein interactions (PPIs) that have been developed to provide simple and direct ways to study PPIs in any living cell, multicellular organism, or in vitro. PCAs can be used
Stephen W Michnick et al.
Methods in enzymology, 470, 335-368 (2010-10-16)
Protein-fragment complementation assays (PCAs) are a family of assays for detecting protein-protein interactions (PPIs) that have been developed to provide simple and direct ways to study PPIs in any living cell, multicellular organism or in vitro. PCAs can be used

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