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重要文件

CN402-05

Sigma-Aldrich

Canine Chondrocytes: CnC (Cryovial)

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About This Item

分類程式碼代碼:
41106514
NACRES:
NA.81

生物源

canine articular cartilage (normal)

包裝

pkg of 500,000 cells

製造商/商標名

Cell Applications, Inc

增長模式

Adherent

染色體組型

2n = 78

形態學

chondrocyte

技術

cell culture | mammalian: suitable

相關疾病

arthritis

運輸包裝

dry ice

儲存溫度

−196°C

一般說明

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Canine Chondrocytes (CnC) are derived from normal canine articular cartilage where they produce and maintain the extracellular matrix of cartilage. Following monolayer culturing, CnC can be grown in alginate microspheres to study chondrocyte proliferation and metabolism in a three-dimensional in vitro system. Alginate microspheres provide more physiological conditions for the chondrocytes and allow them to sustain normal proteoglycans production and retention. Because of the prevalence of degenerative joint disease in geriatric dogs, CnC can be a useful tool for veterinary practice.

Canine Chondrocytes (CnC) were utilized to evaluate anti-inflammatory and anti-oxidant activity of Glyco-Flex III (Yáñez, 2008) and trans-pterostilbene (Remsberg, 2008). They were also used in material studies aimed to develop optimal surfaces/scaffolds for engineered tissues, cellular arrays, biosensors and lab-on-chip devices (Wright, 2012; Shaik 2013a,b).

細胞系來源

Cartilage

應用

production and maintenance of extracellular matrix, cartilage, collagen, differentiation and de-differentiation, signal transduction, apoptosis, differentiation, drug screening, gene expression, cytokine production, agarose assays, chondrocyte adhesion to medical implants, scaffolds for cartilage regeneration

成分

Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO

準備報告

  • 1st passage, >500,000 cells in Canine Chondryocyte Basal Medium containing 10% FBS & 10% DMSO
  • Can be cultured at least 10 doublings

例行更新培養

Please refer to the CnC Culture Protocol.

儲存類別代碼

11 - Combustible Solids

水污染物質分類(WGK)

WGK 3

閃點(°F)

Not applicable

閃點(°C)

Not applicable


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