CMC0022

OverExpress^™ C43(DE3)电感受态细胞

for the highest protein expression

• 分類程式碼代碼: 12352200
• NACRES: NA.85

屬性

• 生物源: Escherichia coli
• 等級: for molecular biology
• 增長模式: adherent or suspension
• 形態學: rod shaped
• 技術: microbiological culture: suitable
• 細胞轉化: competent cell type: electrocompetent; transformation efficiency: ≥1 x 10⁹ cfu/μg
• 運輸包裝: dry ice
• 儲存溫度: −70°C

描述

一般說明

OverExpress^™ Electrocompetent and Chemically Competent Cells are E. coli strains that are effective in expressing toxic proteins from all classes of organisms, including eubacteria, yeasts, plants, viruses, and mammals. The effectiveness of these new strains in expressing toxic proteins has been validated in more than 350 publications. The OverExpress^™ strains contain genetic mutations phenotypically selected for conferring tolerance to toxic proteins. The strain C41(DE3) was derived from BL21(DE3). This strain has at least one mutation, which prevents cell death associated with expression of many recombinant toxic proteins. The strain C43(DE3) was derived from C41(DE3) by selecting for resistance to a different toxic protein and can express a different set of toxic proteins to C41(DE3). OverExpress^™ C41(DE3)pLysS and C43(DE3)pLysS also carry a chloramphenicol-resistant plasmid that encodes T7 lysozyme, which is a natural inhibitor of T7 RNA polymerase. Cells containing pLysS produce a small amount of T7 lysozyme. These strains are used to suppress basal expression of T7 RNA polymerase prior to induction, thus stabilizing recombinants encoding particularly toxic proteins.

Genotype

F – ompT hsdSB (rB- mB-) gal dcm (DE3)

特點和優勢

• Express genes cloned into any T7 vector with these BL21(DE3) derivatives
• Effective in expressing toxic & membrane proteins
• Cited in over 350 research articles

成分

• OverExpress C43(DE3) electrocompetent cells
• pUC 19 transformation control DNA
• recovery medium for expression

法律資訊

OverExpress is a trademark of Imaxio, S.A.

安全資訊

• 儲存類別代碼: 10 - Combustible liquids