MFE-296

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 98031101, human endometrium, Epithelial

• 同義詞: MFE296 Cells
• 分類程式碼代碼: 41106514

屬性

• 生物源: human endometrium
• 包裝: tube of 5 μg 98031101-DNA-5UG; pkg of vial of cells 98031101-1VL
• 增長模式: Adherent
• 染色體組型: Near Tetraploid
• 形態學: Epithelial
• 產品: Not specified
• 受體: Androgen receptor
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human caucasian endometrial adenocarcinoma

細胞系描述

MFE-296 was obtained from a moderately differentiated human endometrial adenocarcinoma. The cells were shown to express functionally intact androgen receptors. Proliferation was inhibted by progestin R5020 and dihydrotestosterone (DHT), the latter one being antagonised by the antiandrogen Casodex. MFE-296 express vimentin and the cytokeratins 7, 8, 18 and 19. Karyotype analysis revealed near tetraploidy for most cells. Further more, the cells have been shown to be tumorigenic in nude mice.

應用

Tumour biology studies, receptor analysis

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 8,13
D16S539: 11,14
D5S818: 11,13
D7S820: 8,10
THO1: 6,9.3
TPOX: 8
vWA: 14,18

培養基

MEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 i.e. seeding 1-2 x 10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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