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Key Documents

L-41(HeLa derivative)

96121716

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About This Item

分類程式碼代碼:
41106514

生物源

human cervix

增長模式

Adherent

染色體組型

Not specified

形態學

Epithelial-like

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human cervix carcinoma

細胞系描述

The cell line L41 was reported to have been established by cloning the line J96, which was originally derived from the bone marrow of a child patient with leukaemia. However, it has been found to be indistinguishable from Hela by STR PCR DNA profiling.Therefore, the cell line should be considered as being derived from HeLa. The cell line L41 was mycoplasma eradicated at ECACC. Ethnicity: Black.

培養基

RPMI 1640 or DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

其他說明

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