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Key Documents

NCI-H69/LX10

96042331, human lung, Not specified

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About This Item

分類程式碼代碼:
41106514

product name

NCI-H69/LX10, 96042331

生物源

human lung

增長模式

Aggregates in suspension

染色體組型

Not specified

形態學

Not specified

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Human small cell lung cancer, drug-resistant

細胞系描述

The drug-resistant cell line NCI-H69/LX10 has been derived from the parent line NCI-H69 (ECACC No 91091802). It is recommended to culture the cells without drug upon resuscitation; add doxorubicine once growth is fully established.

應用

Drug-resistance studies

培養基

RPMI 1640 + 2mM Glutamine + 1.0 μg/ml doxorubicine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 3-9 x 100,000 cells/ml; 5% CO2, 37ºC; resuscitate in drug-free medium and add drug after growth has been established.

其他說明

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分析證明 (COA)

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