推薦產品
product name
Hepa-1c1c7, 95090613
生物源
mouse liver
增長模式
Adherent
染色體組型
Not specified
形態學
Epithelial
產品
Not specified
受體
Aryl hydrocarbon
技術
cell culture | mammalian: suitable
相關疾病
cancer
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Mouse hepatoma
細胞系描述
Hepa-1c1c7 was cloned from the cell line Hepa-1cl which was obtained from Hepa-1. Hepa-1 was derived from the BW7756 hepatoma that developed in a C57L mouse. Hepa-1c1c7 cells express the aryl hydrocarbon (Ah) receptor and were shown to be highly inducible for cytochrome P450IA1. A variety of mutants from this cell line have been established.
應用
Study of Ah receptors, P450IA1 regulation
培養基
α MEM without nucleosides + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-2x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他說明
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分析證明 (COA)
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