AFRC 17D1

94120825, Application: Fusion partner for hybridoma production

• 分類程式碼代碼: 41106514

屬性

• 生物源: Horse x Mouse/Horse Hybridoma
• ImmunoDon: Horse peripheral lymphocytes
• 骨髓瘤: NS0
• 反應: Application: Fusion partner for hybridoma production
• 技術: cell culture | mammalian: suitable
• 同型: Not specified
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Horse peripheral blood lymphocytes x Mouse NS0

細胞系描述

The heterohybridoma AFRC 17D1 was obtained by fusion of lymphocytes from ponies immunised with the influenza A equine 2 virus (isolateA/Equine/Newmarket/79 (H3N8)) with mouse myeloma NS0 cells. The hybridoma was made aminopterin-sensitive by selective growth in 8-azaguanine. This non-Ig-secreting cell line was used as fusion partner for the establishment of equine-mouse heterohybidomas.

免疫原

Influenza A equine 2 virus

應用

Fusion partner for hybridoma production

培養基

RPMI 1640 + 2mM glutamine + 1mM Sodium Pyruvate (NaP) + 5% Foetal Bovine Serum (FBS)

例行更新培養

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C

其他說明

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