MIN human cell line

940822102, IHW Number: 9198

• 分類程式碼代碼: 41106514

屬性

• 生物源: human blood
• 種族: Indonesian
• 性別: not specified
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblastoid
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice

描述

一般說明

Consanguineous: No

Homozygous: No

細胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA分析

HLA DRB1: 1202
HLA DRB3: 301
HLA DQAP: 1.2 4.2
HLA DQA1: 0102 0601
HLA DQB1: 0301 0502
HLA DPB1: 0401 2101

血清學資料

HLA A: 2
HLA A ief:
HLA C: 1 3
HLA B: 15

培養基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

例行更新培養

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他說明

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