推薦產品
product name
PORTHOS, 93122323
生物源
Porcine thyroid
增長模式
Adherent
染色體組型
Not specified
形態學
Epithelial
產品
Thyroglobulin, plasminogen activator (u-PA)
受體
Thyrotropin, insulin, Insulin Growth Factor (IGF-1), hydrocortisone
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
細胞系來源
Porcine thyroid epithelial
細胞系描述
Derived from porcine thyroid. The cells have been adapted to grow in low serum (0.1%) media.
應用
Regulation of thyroid cell metabolism
培養基
Click:RPMI (1:1) + 10μg/ml Insulin + 10mM Hydrocortisone + 5μg/ml Transferrin + 10ng/ml glycyl-L-histydyl-L-lysine acetate + 10ng/ml somatostatin + 10mU/ml Thyroid Stimulating Hormone (TSH) + 2mM Glutamine + 0.1% Foetal Bovine Serum (FBS).
例行更新培養
Split sub-confluent cultures (70-80%) 1:2 seeding at 2 x 10 4 cells/cm2 using 0.05% trypsin/0.025% EDTA in Puck F saline; 2.5% CO2; 37°C.
其他說明
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分析證明 (COA)
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