CALU 1

93120818, human lung, Epithelial

• 同義詞: Calu1 Cells
• 分類程式碼代碼: 41106514

屬性

• product name: CALU 1, 93120818
• 生物源: human lung
• 增長模式: Adherent
• 染色體組型: Hypotriploid, modal no. 62. No Y chromosome.
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian lung epidermoid carcinoma

細胞系描述

Derived from a 47 year old Caucasian male with an epidermoid carcinoma of the lung (grade III). The line will form epidermoid carcinomas in nude mice. Tumours also develop in steroid treated hamsters. Ultrastructure consists of numerous microvilli, prominent rough endoplasmic reticulum, lysosomes, lipid inclusions and no virus particles. The HLA profile is: A-10,11 B15, w35. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.

應用

Tumourigenicity studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 11,12
D16S539: 11
D5S818: 10,12
D7S820: 9,10
THO1: 9.3
TPOX: 8
vWA: 15,16

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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