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Key Documents

C1300 CLONE NA

93120817, mouse nerve, Neuronal-like

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About This Item

分類程式碼代碼:
41106514

product name

C1300 CLONE NA, 93120817

生物源

mouse nerve

增長模式

Adherent

染色體組型

Not specified

形態學

Neuronal-like

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Mouse neuroblastoma

細胞系描述

CLONE NA is a subclone of Neuro-2a, a clonal line of C1300 mouse neuroblastoma cells. The cells were selected for spontaneous mutations deficient in HGPRT in the presence of 8-azaguanine. This cell line has been mycoplasma eradicated at ECACC.

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Cultures may not form a complete monolayer before subculture is required.

其他說明

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分析證明 (COA)

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