ND-C

92090913, mouse nerve, Neuronal

• 分類程式碼代碼: 41106514

屬性

• product name: ND-C, 92090913
• 生物源: mouse nerve
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Neuronal
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse neuroblastoma x Rat neurone hybrid

細胞系描述

ND-C hybrid cells were derived after fusion of primary neonatal-rat dorsal-root-gauglion (DRG) neurons with N18Tg2, a mouse neuroblastoma (C1300) derived azaguanine resistant line. HAT sensitive hybrids were selected and cloned by limiting dilution. The cells express rat Thy 1.1 marker and the sensory neuropeptide substance P. The cell line has been mycoplasma eradicated at ECACC.

應用

Study of neuronal differentiation, cell adhesion and expression of neuronal proteins.

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 1-3 x 10,000 cells/cm²; aspirate or gently tap cells loose, use of trypsin not necessary; 5% CO₂; 37°C.

其他說明

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