C0013

92031001, from human blood

• 分類程式碼代碼: 41106514

屬性

• 生物源: human blood
• HRC Panel 號碼: 3
• 種族: UK Caucasian
• 性別: female
• 增長模式: Suspension
• 染色體組型: Not Specified
• 形態學: Lymphoblastoid
• 技術: cell culture | mammalian: suitable

描述

細胞系來源

UK Caucasian Female

細胞系描述

A B-lymphoblastoid cell line derived from a randomly selected Caucasian blood donor whose parents and grandparents were born in the UK or Ireland. The cell line was generated by Epstein-Barr Virus (EBV) transformation of peripheral blood lymphocytes.

This cell line is part of the European Collection of Cell Cultures (ECACC) Human Random Control (HRC) collection. The HRC collection consists of more than 700 B-lymphoblastoid cell lines derived from randomly selected Caucasian blood donors whose parents and grandparents were born in the UK or Ireland. The HRC collection can be used as a renewable control population for genetic research.

應用

As part of the Human Random Control collection this cell line can be used as a renewable source of genomic DNA reference material for a wide range of genetic research applications.

培養基

RPMI 1640 (R0883) + 2 mM L-Glutamine (G7513) + 20% FBS / FCS (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

例行更新培養

Maintain cultures between 3 x 10⁵ - 2 x 10⁶ cells/ml; 5% CO₂, 37 °C.

其他說明

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