A2058

91100402, human skin (lymph node metastasis), Epithelial

• 同義詞: A2058 Cell Line
• 分類程式碼代碼: 41106514
• 生物源: human skin (lymph node metastasis)
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Nerve growth factor (NGF), Laminin
• 相關疾病: metastasis

屬性

• 產品名稱: A2058, 91100402
• 生物源: human skin (lymph node metastasis)
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Nerve growth factor (NGF), Laminin
• 技術: cell culture | mammalian: suitable
• 相關疾病: metastasis
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human caucasian metastatic melanoma

細胞系描述

This cell line has been isolated from a lymph node metastasis from a 43 year old male patient with malignant melanoma. Due to their highly invasive properties, the cells can be used as a source of cellular invasion associated proteins e.g. collagen type IV collagenase, tissue inhibitor of metalloproteinase-2 and autocrine motility factor.

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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