NCTC 4093

90102530, mouse embryo, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: NCTC 4093, 90102530
• 生物源: mouse embryo
• 增長模式: Adherent
• 染色體組型: Modal no. 71-73; hypotetraploid
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse C57B1/KaLw embryo

細胞系描述

Derived by cloning from a parent line produced from normal mouse embryo tissue (C57B1/KaLw). The cells have spontaneously transformed in vitro. The cells are negative for polyoma virus infection but are susceptible to the virus.

應用

Transformation and virus studies: Polyoma virus

培養基

NCTC 135 100% + 2mM Glutamine.

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using mechanical methods to dislodge the cells (e.g. scraping); 5% CO₂; 37°C.

其他說明

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