推薦產品
product name
RTG-2, 90102529
生物源
fish ovary
增長模式
Adherent
染色體組型
2n = 60
形態學
Fibroblast
產品
Not specified
受體
Not specified
技術
cell culture | mammalian: suitable
運輸包裝
dry ice
儲存溫度
−196°C
相關類別
細胞系來源
Trout Rainbow gonad tissue
細胞系描述
From pooled male/female gonad tissue of yearling rainbow trout (Oncorhynchus mykiss). First cell line to be established from cold-blooded vertebrates. 8-fold difference in growth rate in temperature range 4-24°C. Susceptible to infectious pancreatic necrosis virus, Egtved virus and arboviruses (EEE, VEE).
應用
Virus studies: Inf. pacr. necrotic virus, Egtved virus, arboviruses (EEE, VEE)
培養基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
例行更新培養
Passage using 0.25% trypsin below 24°C. Cells may be seeded as few as 3x10,000 cells/ml 5% CO2; 24°C. Fish cell lines detach easily during transit if the culture is very youn
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