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Key Documents

RR1022

90102526, rat unknown/unspecified, Epithelial

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About This Item

分類程式碼代碼:
41106514

product name

RR1022, 90102526

生物源

rat unknown/unspecified

增長模式

Adherent

染色體組型

2n = 42

形態學

Epithelial

產品

Not specified

受體

Not specified

技術

cell culture | mammalian: suitable

相關疾病

cancer

運輸包裝

dry ice

儲存溫度

−196°C

細胞系來源

Rat Amsterdam Schmidt-Ruppin sarcoma

細胞系描述

Derived from a Rous sarcoma virus (Schmidt-Ruppin strain) induced tumour in an inbred Amsterdam rat. The cells are highly tumorigenic in new-born rats and also chickens. There is some evidence that the cell line is latently infected with the virus.

應用

Tumourigenicity and virus studies: Rous sarcoma virus

培養基

Medium 199 (HBSS) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

其他說明

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