SIRC

89090404, rabbit eye, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: SIRC, 89090404
• 生物源: rabbit eye
• 增長模式: Adherent
• 染色體組型: 2n = 44
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rabbit Cornea

細胞系描述

Derived from the cornea of a normal rabbit. The line is suitable for primary isolation of rubella virus in addition to propagation and assay. The cells are not susceptible to poliovirus 2, coxsackie A9 and B5.

應用

Virus studies: Rubella virus

培養基

EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4 x 10,000 cells/cm² using 0.25% trypsin 0.02% EDTA; 5% CO₂, 37°C.

其他說明

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