OX-35 Cell Line from murine myeloma

clone 5, Reactivity: Anti- CD4, rat, 89041184

• 分類程式碼代碼: 41106514

屬性

• 生物源: Mouse x Mouse Hybridoma
• 無性繁殖: 5, monoclonal
• 包裝: tube of 5 μg 89041184-DNA-5UG; pkg of vial of cells 89041184-1VL
• ImmunoDon: BALB/c mouse spleen
• 骨髓瘤: NS0/1
• 反應: Anti-CD4, rat
• 技術: cell culture | mammalian: suitable
• 同型: IgG2a
• 運輸包裝: dry ice

描述

細胞系來源

Mouse NS0/U x Mouse BALB/c spleen

細胞系描述

The antibody is directed against the CD4 antigen on rat T cells and macrophages. The specificity is the same as that of W3/25, though their binding is non-competitive. OX-35 will compete with OX-36.

免疫原

T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen

培養基

RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO₂; 37°C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.