R2C

89031606, rat testis, Epithelial

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: R2C, 89031606
• 生物源: rat testis
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Steroid hormones; 20-hydroxypregn-4-en-3-one; progesterone
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Rat testis Leydig cell tumour

細胞系描述

A rat testis Leydig cell tumour cell line derived from a 2-month-old male WFu rat. Virus susceptibility: herpes simplex; vaccinia; pseudorabies; vesicular stomatitis (Indiana). Resistant to poliovirus 2. Stimulation by other hormones or cAMP is not required for hormone secretion.

培養基

HAMS F10 + 2mM Glutamine + 15% Horse serum + 2.5% Foetal Bovine Serum (FBS). Do not use heat-inactivated serum.

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA;5% CO₂; 37°C.

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