EHM human cell line

88052079, IHW Number: 9080

• 分類程式碼代碼: 41106514

屬性

• 生物源: human blood
• 種族: German
• 性別: male
• 增長模式: Suspension
• 染色體組型: Not specified
• 形態學: Lymphoblastoid
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice

描述

一般說明

Consanguineous: No

細胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

DNA分析

A: 301
Cw: 401
B: 3501 3503
HLA DRA: 101
HLA DRB1: 101
HLA DRB6: 1
HLA DQA1: 101
HLA DQB1: 501
HLA DPA1: 1
HLA DPB1: 0401 0402

血清學資料

HLA A: 3
HLA A ief: 3.2
HLA C: 4
HLA B: 35
HLA B ief: 35.3
Bw4/bw6: 6
HLA Dw: 1
HLA DR: 1
HLA DQ: 5
HLA DP: 4

培養基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

例行更新培養

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

免責聲明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.