KNRK

87061001, rat kidney, Epithelial-like

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: KNRK, 87061001
• 生物源: rat kidney
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial-like
• 產品: Growth factors
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Normal rat kidney, Kirsten MSV transformed

細胞系描述

Derived from normal rat kidney transformed by Kirsten sarcoma virus. Cells do not produce virus but carry the virus genome. In serum free medium the cell line produces growth factors capable of stimulating quiescent cells to grow in monolayers or in soft agar.

應用

Transformation, growth factors and virus studies: Kirsten MSV

培養基

DMEM + 2mM Glutamine + 5-10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 i.e. seeding at 1-3x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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