Ehrlich-Lettre Ascites strain E cell line

87032503, mouse unknown/unspecified, Epithelial

• 分類程式碼代碼: 41106514

屬性

• product name: Ehrlich-Lettre Ascites strain E cell line, from mouse unknown/unspecified, 87032503
• 生物源: mouse unknown/unspecified
• 增長模式: Adherent
• 染色體組型: 2n = 40
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse carcinoma

細胞系描述

Established from the explant of a 7 day old tumour from the parental Ehrlich-Lettre Ascites carcinoma. Prior to development, strain E was subjected to a schedule of seven mouse passages and six in vitro passages over a period of 17 months. The cells exhibit an unusually high mean chromosome number and possess the marker chromosomes present in the parental Ehrlich tumour. This cell line may be of use in comparative testing of anti-tumour agents in vivo and in vitro.

應用

Anti-tumour test and tumourigenicity studies

培養基

NCTC 135 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm²using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Media change 3 times a week.

其他說明

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