LS180

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 87021202, human colon, Epithelial-like

• 分類程式碼代碼: 41106514

屬性

• 生物源: human colon
• 包裝: tube of 5 μg 87021202-DNA-5UG; pkg of vial of cells 87021202-1VL
• 增長模式: Adherent
• 染色體組型: Modal no. 45 (2n=46)
• 形態學: Epithelial-like
• 產品: Carcinoembryonic antigen (CEA), Mucin
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human Caucasian colon adenocarcinoma

細胞系描述

Derived from a 58 year old female Caucasian with Dukes type B adenocarcinoma of the colon. The cells secrete CEA and mucin and are tumorigenic in nude mice. LS174T (Sigma Catalogue number. 87060401) was derived from the same patient. The cells have not been exposed to trypsin.

應用

Tumourigenicity studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-5x10,000 cells/cm² by scraping; 5% CO₂; 37°C.

其他說明

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