NIH-3T3 4-2

86041101, mouse skin, Fibroblast

• 分類程式碼代碼: 41106514

屬性

• product name: NIH-3T3 4-2, 86041101
• 生物源: mouse skin
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

一般說明

Product has lost contact inhibition

細胞系來源

Mouse fibroblast

細胞系描述

A mouse fibroblast cell line which has an integrated mutant Moloney MuLV. The cell line can be used to generate helper-free defective retroviruses.

應用

Virus studies: Moloney MuLV, retroviruses

培養基

DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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