MiCL1 (S+L-)

86020603, mink lung, Fibroblast-like

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: MiCL1 (S+L-), 86020603
• 生物源: mink lung
• 包裝: tube of 5 μg 86020603-DNA-5UG; pkg of vial of cells 86020603-1VL
• 增長模式: Adherent
• 染色體組型: 2n = 30
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mink lung, Moloney murine sarcoma virus infected

細胞系描述

A clone of the mink lung cell line Mv.1.Lu (Sigma Catalogue number. 88050503). Isolated from a single cell of a focus produced in the monolayer after infection with Moloney murine sarcoma virus associated with the RD-114 helper leukaemia virus. Super infection with non-transforming leukaemia virus results in transformation and net amplification of sarcoma-specific RNA sequences.

應用

Virus studies: Moloney MSV and RD-114 helper leukaemia virus Leukemogenesis

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 4x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.