BW5147.G.1.4.OUA/R.1

85082302, mouse blood, Lymphoblast

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: BW5147.G.1.4.OUA/R.1, 85082302
• 生物源: mouse blood
• 包裝: tube of 5 μg 85082302-DNA-5UG; pkg of vial of cells 85082302-1VL
• 增長模式: Suspension
• 形態學: Lymphoblast
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

一般說明

For complete product information, please see BW5147.G.1.4.OUA/R.1

細胞系來源

Mouse AKR/J T cell lymphoma

細胞系描述

This line is a mutant sub-line of BW5147 which was derived from an AKR/J mouse lymphoma. The cells are resistant to 100μM thioguanine and 1mM ouabain. The cells can be used to produce T-cell hybrids.

應用

Fusion partner

培養基

SMEM + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 2mM Sodium Pyruvate (NaP) + 0.05mM 2-Mercaptoethanol (2ME) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Maintain cultures between 1-6x100,000 cells/ml; 5% CO₂; 37°C.

其他說明

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