F9

85061803, mouse embryo, Not specified

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: F9, 85061803
• 生物源: mouse embryo
• 包裝: tube of 5 μg 85061803-DNA-5UG; pkg of vial of cells 85061803-1VL
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Not specified
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse 129 embryo teratocarcinoma

細胞系描述

Derived from testicular carcinoma. F9 undergo very limited differentiation under normal conditions but can be induced to differentiate into parietal endoderm in the presence of retinoic acid and dibutyryl cAMP.

應用

Differentiation studies

培養基

DMEM + 2mM Glutamine + 10-15% Foetal Bovine Serum (FBS). Pre-soak flasks in 0.1% gelatin for over 2 hours and wash with PBS.

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂, 37°C. Upon resuscitation, cells shed large quantities of debris, leave for 2 days before subculture. Please note: Culture v

其他說明

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