C57/B1

85011438, mouse skin, Epithelial

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: C57/B1, 85011438
• 生物源: mouse skin
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse C57BL/6J melanoma epithelial

細胞系描述

Spontaneous tumour of C57 black/6J mouse originally obtained from Dr Parrish of Australian National University, Canberra. Rendered HGPRT- by selection in 20μg/ml 6-thioguanine.

應用

Tumourigenicity studies

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) at a maximum dilution of 1:10 i.e. seeding at 2-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Monolayers will detach if monolayer becomes over-confluent.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.