HF19

85011418, human lung, Fibroblast-like

• 分類程式碼代碼: 41106514

屬性

• product name: HF19, 85011418
• 生物源: human lung
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fibroblast-like
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human foetal lung fibroblast

細胞系描述

HF19 has been established from a 14 week old female foetus. The cells are heterozygous for thymidine kinase and have been used for studies of radiation-induced DNA damage.

應用

DNA repair studies

DNA分析

STR-PCR Data: Amelogenin: X
CSF1PO: 10,13
D13S317: 9,13
D16S539: 13,14
D5S818: 11,12
D7S820: 11,12
THO1: 6,7
TPOX: 8,11
vWA: 14,18

培養基

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-4x10,000 cells /cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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