OX-96

13082216, from Mouse x Rat Hybridoma

• 分類程式碼代碼: 41106514

屬性

• 生物源: Mouse x Rat Hybridoma
• ImmunoDon: Rat PVG spleen
• 骨髓瘤: NS1
• 反應: Mouse CD22
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系描述

The hybridoma cell line OX-96 secretes monoclonal IgG1 antibodies reactive with mouse CD22. OX-96 reacts with domain 1 but does not block ligand binding.

免疫原

Recombinant CD22

應用

The isotype of the monoclonal antibody produced by this cell line is rat IgG1.

培養基

RPMI 1640 + 2mM Glutamine + 10% Fetal Bovine Serum (FBS)

例行更新培養

Seed at 5 x 10⁴ cells/ml (5 x 10⁵ cells/ml at resuscitation). Subculture when cells reach 5 x 10⁵ - 1 x 10⁶ cells/ml (usually after 3-4 days), 5% CO₂, 37°C. 20% FBS can be used at resuscitation.

其他說明

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.