FM-95

13012425, human skin, Fibroblastic

• 分類程式碼代碼: 41106514

屬性

• product name: FM-95, 13012425, from human skin
• 生物源: human skin
• 描述: Human melanoma, metastasis
• 增長模式: Adherent
• 形態學: Fibroblastic
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系描述

FM-95 (ESTDAB-034) was established from a metastatic cutaneous malignant melanoma of a male patient. The FM-95 cell line is one of more than 170 characterized melanoma cell lines collected during the ESTDAB project (European Searchable Tumour Line Database) part of the European Commissions fifth framework infrastructures programme (contract no. QLRI-CT-2001-01325). ESTDAB contains information on HLA genotype and surface expression of HLA molecules, expression of tumour antigens, antigen processing capability, production of and response to cytokines and chemokines, apoptosis regulation and expression of adhesion/accessory molecules. Such information allows researchers to identify cells possessing specific parameters important for studies of immunity, immunogenetics, gene expression, metastasis, response to chemotherapy, and other tumour biological experimentation.

DNA分析

STR-PCR Data:

Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 9,10
D16S539: 9,13
D5S818: 12,13
D7S820: 8,11
THO1: 9.3,9.3
TPOX: 8
vWA: 15,18

培養基

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

例行更新培養

Split sub-confluent cultures (70-80%) seed new cultures at 2-4 x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37°C. Saturation density approx 8 x10,000 cells/cm²

其他說明

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Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.