MM466

12070507, human skin (metastasis), Epithelial

• 同義詞: MM 466
• 分類程式碼代碼: 41106514

屬性

• 產品名稱: MM466, 12070507
• 生物源: human skin (metastasis)
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Epithelial
• 受體: Not specified
• 相關疾病: metastasis

描述

細胞系來源

Human melanoma metastatic site lymph node

細胞系描述

mM466 is a human melanoma derived from a metastatic site (lymph node). Cell Bank Australia reference number CBA-1353.

DNA分析

Not specified

培養基

RPMI 1640 (R0883) (with 2 mM L-Glutamine (G7513) + 25mM HEPES) + 10% FBS.

例行更新培養

Optimal split ratio 1:2-1:4 (seeding density 1.4 x 10⁴ cells/cm²). Cells incubated at 37 °C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37 °C for 5 min. Medium change twice weekly. The cell line MM466 may take up to 72 hours to recover from thaw.

其他說明

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Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Depositor and originator: Peter Parsons - Queensland Institute of Medical Research, Australia