LIM1899 cell line human

12062001

• 同義詞: LIM 1899, LIM-1899
• 分類程式碼代碼: 41106514

屬性

• 生物源: human colon
• 增長模式: adherent
• 形態學: Epithelial; cells are small rounded and grow in clusters
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系描述

Columnar cell adenocarcinoma of the colon. Cell Bank Australia reference number: CBA0163.

培養基

RPMI-1640 (with 2mM L-Glutamine + 25mM HEPES) + 10% FCS, Insulin 0.6 μg/ml, Hydrocortisone 1 μg/ml, 1-Thioglycerol 10 μM

例行更新培養

Optimal split ratio 1:8 (seeding density 1 x 10⁴ cells/cm²). Cells incubated at 37°C with 5% CO₂. Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min. LIM1899 requires the growth medium to be changed 3 times each week.

其他說明

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