GPNT

9061601, rat brain, Fusiform

• 分類程式碼代碼: 41106514

屬性

• 產品名稱: GPNT, from rat brain, 09061601
• 生物源: rat brain
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Fusiform
• 產品: GPNT cells express tight junction proteins
• 受體: P-glycoprotein, PECAM-1, ICAM-1, VCAM-1 Glut-1, transferrin receptor, MHC class I
• 技術: cell culture | mammalian: suitable
• 運輸包裝: dry ice

描述

細胞系來源

An immortalised Lewis rat brain vascular endothelial cell line

細胞系描述

GPNT was derived from the immortalised rat brain endothelial cell line GP8 by lipofectin-mediated transfection with a vector containing the puromycin resistance gene. GPNT exhibits a non-transformed phenotype.

DNA分析

Not specified

培養基

Hams F10 + 10% Heat-inactivated FCS + 2 mM Glutamine + 2 ng/ml basic fibroblast growth factor + 80 μg/ml heparin. All plasticware is pre-coated for several hours with collagen (Type I from calf skin) at 10μg/cm2 from a 0.01% solution in acetic acid. After removal the coated plasticware is exposed to a mMonia vapour for 5 min. Plasticware must be rinsed thoroughly with PBS prior to use.

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 10hrs.

其他說明

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免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.