328/14

8062541, mouse nerve (fibroblast), Neuronal/fibroblastic

• 分類程式碼代碼: 41106514

屬性

• product name: 328/14, 08062541
• 生物源: mouse nerve (fibroblast)
• 增長模式: Adherent
• 染色體組型: Modal No. varied with passage see reference
• 形態學: Neuronal/fibroblastic
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Mouse neuroblastoma x mouse L cell fibroblast hybrid

細胞系描述

Hybrid generated by fusion of 6-thioguanine-resistant mutant of mouse neuroblastoma N4TG3 (Sigma Catalogue number 08062515) and 8-bromodeoxyuridine-resistant mutant of mouse L cell fibroblast line B82 (Sigma Catalogue number 08062522) using inactivated Sendai virus. The fusion of N4TG3 x B82 generated two types of hybrid cells: neuronal resembling the neuroblastoma and epithelioid with clearly visible nucleus. 328/14 is of the neuronal type.

應用

Used as a neuronal Model

培養基

DMEM + 2 mM glutamine + 10% Foetal Bovine Serum (FBS) + HAT (0.1 mM hypoxanthine, 10 μM aminopterin and 16 μM thymidine) Cells can be cultured without HAT provided they were cultured in between in HT medium for 3-4 days

例行更新培養

Split sub-confluent cultures (70-80%) 1:4 to 1:8 i.e. seeding at 2-4x10,000 cells/cm² using 0.05% trypsin/EDTA; 5% CO₂; 37°C.

其他說明

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