ANGM-CSS

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 8040401, human brain, Fusiform, can form heaps of cells

• 分類程式碼代碼: 41106514

屬性

• 生物源: human brain
• 包裝: tube of 5 μg 08040401-DNA-5UG; pkg of vial of cells 08040401-1VL
• 增長模式: Not specified
• 染色體組型: Near tetraploid; range 88 to 91 - see Cell Line Description for more information
• 形態學: Fusiform, can form heaps of cells
• 產品: Not specified
• 受體: Not specified
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human glioblastoma

細胞系描述

A human A human glioblastoma multiforme cell line derived from a 65-year-old male. The results of immunocytochemical analysis for GFAP, Vimentin, NestiN and CD33 were positive in early and late passage cells. MGMT methylation was negative. Karyotype: Near tetraploid; range 88 to 91 with two copies of marker: der(6;7;12;)del(6q ), t(6;11), t(6;17) del(17q). Six copies of chromosome 7 and other rearrangements with loss and gain of chromosome fragments involving chromosomes 1,2,3,5,10,9,19,20. The X and Y chromosomes are present. (Information provided by Depositor)

培養基

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS)

例行更新培養

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-4x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂;37ºC.

其他說明

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