H357

6092004, human mouth, Polygonal

• 分類程式碼代碼: 41106514

屬性

• product name: H357, 06092004
• 生物源: human mouth
• 增長模式: Adherent
• 染色體組型: Not specified
• 形態學: Polygonal
• 產品: Not specified
• 受體: TGFbeta type I receptor, TGFbeta II receptor, TGFbeta III receptor
• 技術: cell culture | mammalian: suitable
• 相關疾病: cancer
• 運輸包裝: dry ice
• 儲存溫度: −196°C

描述

細胞系來源

Human oral squamous cell carcinoma, tongue

細胞系描述

Established from a squamous cell carcinoma (SCC) of the tongue (20mm) of a 74 year-old male patient. STNMP stage I, well differentiated, node negative tumour. Mutant p53, codon 110 exon 4, G to A; the previously reported mutant Ha-ras status of this cell line: codon: 13, G to A, codon 61, A-G is under investigation. This cell line is highly responsive to TGF (Transforming growth factor )-β and undergoes epithelial to mesenchymal transition. It expresses high levels of TGF-β1. Fahey et al (1996). Tumourigenic in athymic nude mice on subcutaneous injection and non-tumorigenic on orthotopic injection. Haplotype information: A*02,A*31; B*40,B*44; Cw*03,Cw*05

培養基

DMEM:HAMS F12 (1:1) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 0.5 ug/ml sodium hydrocortisone succinate

例行更新培養

Split sub-confluent cultures (70-80%), approximately every 5-6 days, 1:8 to 1:10 using 0.05% trypsin/EDTA; 5% CO₂; 37°C. Suggested seeding density 5 x 1000 cells/cm². Cells can take approximately 10 minutes to detach, an alternative is to trypsinise 2

其他說明

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免責聲明

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.